Payment extra fat was counted after the an overnight punctual using heavens-displacement plethysmography (BOD POD Express; Lifestyle Aspect, Concord, California, USA)

Payment extra fat was counted after the an overnight punctual using heavens-displacement plethysmography (BOD POD Express; Lifestyle Aspect, Concord, California, USA)

Sides and you will cool circumferences was basically mentioned towards users during the good relaxed condition position. Waist width was removed in the mid-section between the anterior premium iliac spine together with all the way down boundary of your own rib cage, and you may hip circumference on quantity of the more trochanter. All measurements have been made throughout the studies several months from the an individual observer (Elizabeth. L. Lim).

Analytical tips

Plasma glucose concentration was measured by the glucose oxidase method (YSI glucose analyser; Yellow Springs, OH, USA), plasma insulin and C-peptide concentrations by ELISA (Dako, Ely, UK), plasma triacylglycerol by lipase with released glycerol measured by a Roche Cobas centrifugal analyser using a colorimetric assay (ABX Diagnostics, Montpellier, France) and HbA1c by a Biorad HPLC (TOSOH Corporation, Tokyo, Japan). 2 H atom percent excess in plasma glucose was determined using a Thermo ‘Voyager’ single quadrupole mass spectrometer with Thermo ‘Trace’ gas chromatograph (Thermo Scientific, Waltham, MA, USA).

Analytical procedures

Statistical analyses were performed using SPSS 17.0 software (SPSS Inc., Chicago, IL, USA). “Payment extra fat was counted after the an overnight punctual using heavens-displacement plethysmography (BOD POD Express; Lifestyle Aspect, Concord, California, USA)” の続きを読む